[Method of determining the molar concentration of proteinase inhibitors and their affinity for the enzymes]

This paper describes a graphic method for measuring the affinity and molar concentration of proteinase inhibitors from different sources, using data on the titration of the inhibitor by the enzyme of a known molar concentration. Linearization of experimental data in the (formula; see text) coordinates makes it possible to measure the value Ki with respect to the slope of the curve and to determine the inhibitor concentration with respect to the curve intercept with the ordinate axis. The method can be used to characterize proteinase inhibitors during microbial screening and at certain purification stages.