Behaviour and enzyme release by Anisakis sp. larvae (Nematoda: Ascaridida)

Anisakis sp. larvae when incubated in vitro produced secretions/excretions which released the dye from an azocoll substrate, indicating protease activity. The optimal conditions for enzyme release and activity were a temperature of 37 degrees C and a pH of 7.5. In addition the larvae showed peak behavioural activity at 37 degrees C, the body temperature of the final, marine mammalian host. The results are discussed in relation to their possible significance to the invasion of the various hosts in the life-cycle.