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[Determination of anti-core antibodies with the immunoenzymatic method. Comparison with the radioimmunological
Insights
Enzyme-linked immunosorbent assay (ELISA) and radioimmuno assay (RIA) were compared for Hepatitis B core antibody (HBcAb) detection. ELISA demonstrated comparable accuracy to RIA, with enhanced sensitivity for positive samples and greater ease of use.
Area of Science:
- Hepatitis B diagnostics
- Immunological assays
Background:
- Hepatitis B virus (HBV) infection remains a significant global health concern.
- Accurate detection of Hepatitis B core antibody (HBcAb) is crucial for diagnosing past or present HBV infection.
- Enzyme-linked immunosorbent assay (ELISA) and radioimmuno assay (RIA) are common methods for antibody detection.
Purpose of the Study:
- To compare the diagnostic performance of ELISA and RIA for HBcAb detection.
- To evaluate the sensitivity and ease of use of both assays.
Main Methods:
- A total of 366 serum samples were analyzed for HBcAb.
- Enzyme-linked immunosorbent assay (ELISA) and radioimmuno assay (RIA) were employed for detection.
- Twenty positive samples were titered to assess assay sensitivity.
Main Results:
- Both ELISA and RIA yielded largely concordant results for HBcAb detection.
- RIA presented a few doubtful cases, suggesting lower specificity in certain instances.
- ELISA exhibited greater sensitivity in titrating positive samples compared to RIA.
Conclusions:
- ELISA offers a sensitive and reliable alternative to RIA for HBcAb detection.
- The ELISA method is operationally simpler and requires less specialized equipment than RIA.
- ELISA is recommended as a practical and effective assay for HBcAb screening.
Abstract:
The authors tested 366 samples for the detection of HBcAb using enzyme-linked immunosorbent assay (ELISA) and radioimmuno assay (RIA). Twenty samples were titered to compare the sensitivity of the two tests. The results showed that the two methods gave very similar results, with only a few doubtful cases for RIA. ELISA seems to be more sensitive than RIA for the titration of the positive samples. The authors conclude that the ELISA method is easier than RIA and does not require any apparatus in particular.