Adsorption chromatography of nucleic acids on siliconized porous glass

Bovine liver tRNA, E. coli rRNA or calf thymus DNA adsorbed on siliconized porous glass in 5M NaCl-0.01M Tris-HCl at pH 7.6 were chromatographed by the lowering of NaCl concentration on the columns. tRNAs were eluted at 5-3M NaCl and were separated in the eluting order of Pro, Val, Ile, Thr, Ser and Phe tRNA. RNA species in crude rRNA were also separated. A half part of calf thymus DNA passed through the column in 5M NaCl-0.01M Tris-HCl and the residual adsorbed was eluted at 2-0M NaCl. The DNA adsorbed is larger than the DNA passed through. Recovery of those nucleic acids from the column was 83-100%.