Discontinuous thermotropic response of Tetrahymena membrane lipids correlated with specific lipid compositional...

Steady-state fluorescence polarization measurements of 1,6-diphenyl-1,3,5-hexatriene in microsomal lipids from Tetrahymena pyriformis cells grown at 39 or 15 degrees C revealed discrete slope discontinuities in plots of polarization vs. temperature. Two well-defined 'break points' were present in the 0-40 degrees C temperature range examined and their precise location was dependent upon the growth temperature of the cells. By mixing phospholipids from cells grown at different temperatures, the break points at 17.5 and 32 degrees C in 39 degrees C-lipid multilayer preparations were shown to correlate with the breaks at 12 and 27 degrees C, respectively, in similar preparations from 15 degrees C-grown cells. The discrete break points were also present, but at slightly different characteristic temperatures, in a phosphatidylcholine fraction and a phosphatidylethanolamine plus 2-amino-ethylphosphonolipid fraction purified from the phospholipids and in total microsomal lipids (phospholipids plus the sterol-like triterpenoid, tetrahymanol). However, catalytic hydrogenation of the phospholipid fatty acids or mixing the non-hydrogenated phospholipids with increasing proportions or synthetic dipalmitoyl phosphatidylcholine eliminated the break points. We interpret this discontinuous thermotropic response in microsomal lipids as signalling a lipid phase separation of importance in regulating physiological events.