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Large-scale purification of IgM from human sera. Comparison of three optimized procedures utilizing protein A
Research in Experimental Medicine. Zeitschrift Fur Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie
|January 1, 1980
Abstract:
for the preparation of gram amounts of IgM from human sera sedimentation at 100,000 g or treatment with ZnSO4 of the redissolved "euglobulin"-precipitate was compared to direct precipitation from the clarified serum by boric acid. Three alternative large scale purification procedures were developed, leading to an IgM-sample characterized as pure by various criteria. Inclusion of protein A chromatography proved to enhance the yield very considerably.