Apoflavodoxin aggregation following dissociation of flavin

Flavodoxins were isolated for the cyanobacteria Anacystis nidulans and Nostoc strain MAC, and from the red alga Chondrus crispus, and apoflavodoxins prepared by five methods. Gel electrophoretic studies showed that whereas the apoproteins of A. nudulans and Nostoc strain MAC were recovered in monomeric form, the removal of riboflavin 5'-phosphate from C. crispus flavodoxin resulted in extensive aggregation of the apoprotein. In extent and nature this aggregation differed with the dissociating agent used.