Effect of Aroclor 1254 on leukocyte glucose uptake

Uptake of glucose in human leukocytes was estimated by measuring incorporation of 14C-labeled 2-deoxyglucose (2-DG) into the cells. Exposure of lymphocytes and monocytes, but not neutrophils, to Aroclor 1254 (10 microM) caused a decreased 2-DG uptake. This method of measuring glucose uptake may be useful for detecting potential polychlorinated biphenyl (PCB) target cells.