An anticoagulant for transport of bovine blood

Various anticoagulant were assessed in order to develop a transport media for bovine blood which would improve leukocyte stability. The effects of time, disease in the donor and different personnel and laboratory equipment were also tested. The transport media most effective for leukocyte stability was found to be Titriplex IV. This is a modification of Titriplex I by the inclusion of acetylsalicyclic acid to prevent cellular clumping and by the removal of methylene blue. Anticoagulant citrate dextroseformalin-ASA performed equally well for transport but cellular morphology was impaired. Febrile or neoplastic disease in the donor animal did not alter the efficiency of the formalin containing anticoagulants.