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Related Experiment Videos

Macro creatine kinase: a study on CK-linked immunoglobulin.

H Yuu, S Ishizawa, Y Takagi

    Clinical Chemistry
    |December 1, 1980
    PubMed
    Summary

    Creatine kinase (CK) linked to immunoglobulins, found in 0.8% of cases, can cause false-positive results in diagnostic tests. These CK-immunoglobulin complexes are distinct from other macro-CK forms.

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    Area of Science:

    • Clinical Chemistry
    • Immunology
    • Biochemistry

    Background:

    • Creatine kinase (CK) is a crucial enzyme in energy metabolism.
    • Macro-CK, including CK-immunoglobulin complexes, can interfere with diagnostic assays.
    • Understanding CK-immunoglobulin complexes is important for accurate clinical interpretation.

    Purpose of the Study:

    • To characterize the prevalence and nature of CK-linked immunoglobulins.
    • To investigate the diagnostic implications of these complexes.
    • To differentiate CK-immunoglobulin complexes from other macro-CK entities.

    Main Methods:

    • Enzyme-immunofixation electrophoresis to identify CK-immunoglobulin complexes.
    • Serum dissociation and re-formation studies at varying pH.

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  • Gel filtration and antibody inhibition assays.
  • Urea and heat treatment to assess CK activity.

    • Main Results:

    • CK-linked immunoglobulins were identified in 0.8% of cases studied.
    • Heavy chains were consistently of class alpha, with lambda or kappa light chains.
    • Complexes dissociated at pH 3.4 and reformed at pH 7.4.
    • Residual CK-MM activity observed after urea/heat treatment, unlike normal CK.
    • False-positive CK-B activity noted in immuno-inhibition tests.

    Conclusions:

    • CK-linked immunoglobulins represent a type of circulating immune complex.
    • These complexes must be differentiated from other macro-CK forms.
    • Their presence can lead to erroneous CK-B activity measurements, impacting clinical diagnosis.