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Related Experiment Video

Updated: Jun 23, 2026

Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells

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Published on: June 13, 2019

Active chloride secretion in the normal human jejunum.

G R Davis, C A Santa Ana, S Morawski

The Journal of Clinical Investigation

|December 1, 1980

Summary

The human jejunum actively secretes chloride and water, a process masked by absorption. This active chloride secretion may regulate fluid absorption in the small intestine.

Area of Science:

Gastroenterology
Physiology
Intestinal transport

Background:

The small intestine's absorptive processes can mask fluid secretion.
Active absorption in the human jejunum depends on luminal bicarbonate.

Purpose of the Study:

To determine if the small intestine normally secretes fluid.
To investigate the mechanisms of jejunal fluid and electrolyte transport.

Main Methods:

Perfusing bicarbonate-free solutions in the jejunum of normal subjects.
Measuring fluid and electrolyte secretion and absorption.
Analyzing passive and active transport mechanisms for sodium and chloride.

Main Results:

The jejunum secretes sodium chloride and water when perfused with bicarbonate-free solutions.

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A Fluorescence-Based Assay of Membrane Potential for High-Throughput Functional Study of Two Endogenous Ion Channels in Two Epithelial Cell Lines

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Last Updated: Jun 23, 2026

Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells

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Published on: June 13, 2019

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique

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A Fluorescence-Based Assay of Membrane Potential for High-Throughput Functional Study of Two Endogenous Ion Channels in Two Epithelial Cell Lines

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Published on: June 22, 2022

Sodium secretion is passive, while chloride is actively secreted against an electrochemical gradient.

Observed chloride flux ratios contradicted passive movement calculations.

Conclusions:

The normal jejunum actively secretes chloride, a process usually masked by greater absorption.
Active chloride secretion is a potential regulator of fluid absorption in the human intestine.