[Distribution of proteoglycans in the sclera]

The Authors have studied, with histochemical methods, the manner of proteoglycans distribution in superficial and deep layers of the sclera, which are characterized by structural differences regarding collagen. The pictures, on one hand, are in agreement with biochemical data which indicate the presence of dermatan-sulfate, chondroitin-sulfate and hyaluronic acid, on the other hand, show a marked glycosaminoglycans distribution in the deep layers and a lessening of their distribution relative to the superficiality of the layers. Furthermore alcianophilic particles are visualized at the electron microscope. In the superficial layers such particles are in direct relation, often periodically, with the collagen fibrils, while in the deep layers particles without this direct relationship are also observable. The Authors point out a topographic coincidence between variations regarding the proteoglycans distribution and variations regarding collagen. Moreover, the obtained pictures suggest the existence of a particular proteoglycans-collagen interaction corresponding to the deep layers where fibrillogenesis occurs.