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Related Experiment Videos

Apparent antihaemophilic activity of basic amphoteric polyelectrolytes.

T Exner, K A Rickard, H Kronenberg

    The Australian Journal of Experimental Biology and Medical Science
    |April 1, 1978
    PubMed
    Summary

    Ampholine 9-11, used in isoelectric focusing, shortens clotting time in factor VIII deficient plasma. This effect is linked to contact activation inhibition and shows linear response on double log plots.

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    Area of Science:

    • Biochemistry
    • Hematology

    Background:

    • Prolonged partial thromboplastin clotting time with kaolin (PTTK) indicates factor VIII deficiency.
    • Ampholine 9-11 is an amphoteric polyelectrolyte with an alkaline isoelectric point, typically used for isoelectric focusing.

    Purpose of the Study:

    • To investigate the effect of Ampholine 9-11 on clotting factor deficiencies, specifically factor VIII deficiency.
    • To characterize the mechanism by which Ampholine 9-11 influences plasma clotting time.

    Main Methods:

    • Assessing the impact of Ampholine 9-11 on prolonged PTTK in factor VIII deficient plasma.
    • Analyzing the dose-response relationship of Ampholine 9-11 using double log plots.
    • Evaluating the activity of Ampholine 9-11 on other clotting factors (IX, XI, V, VII, X, II, XII).
    • Investigating the effect of Ampholine 9-11 on contact activators.

    Main Results:

    • Ampholine 9-11 significantly shortened the PTTK in factor VIII deficient plasma.
    • The PTTK response to Ampholine 9-11 exhibited a linear relationship on double log paper.
    • At 2% concentration, Ampholine 9-11 demonstrated activity for factors VIII and IX, and partial activity for factor XI.
    • No significant correction was observed for plasmas deficient in factors V, VII, X, II, or XII.
    • Ampholine 9-11 inhibited low concentrations of contact activators, and its corrective effect on factor VIII deficiency correlated with contact activation levels.

    Conclusions:

    • Ampholine 9-11 possesses procoagulant properties, particularly beneficial in factor VIII deficiency.
    • The mechanism involves inhibition of contact activators, suggesting a role in modulating the intrinsic pathway of coagulation.
    • Further research is warranted to explore its therapeutic potential in specific bleeding disorders.

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