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Related Experiment Videos

A rapid paper-disc test for penicillinase.

K K Tu, J H Jorgensen, C W Stratton

    American Journal of Clinical Pathology
    |April 1, 1981
    PubMed
    Summary

    A new paper disc assay detects penicillinase production by bacteria. This simple, rapid method uses penicillin and phenol red to indicate enzyme activity through a color change.

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    Area of Science:

    • Microbiology
    • Biochemistry
    • Diagnostic Assays

    Background:

    • Penicillinase is an enzyme produced by some bacteria that confers resistance to penicillin antibiotics.
    • Accurate and rapid detection of penicillinase is crucial for effective antibiotic treatment and infection control.
    • Existing methods for detecting penicillinase may be time-consuming or require specialized equipment.

    Purpose of the Study:

    • To develop a practical and accessible acidimetric assay for the detection of penicillinase production.
    • To create a simple, rapid, and accurate method for identifying penicillinase-producing bacteria.

    Main Methods:

    • Paper discs were impregnated with buffered penicillin G and 1% phenol red.
    • Discs were incubated with bacterial suspensions in saline solution.
    • Visual detection of a yellow color change within 1–30 minutes indicated a positive result.

    Main Results:

    • The developed assay successfully detected penicillinase production in bacterial strains.
    • A distinct yellow color change was observed within 1 to 30 minutes for penicillinase-positive samples.
    • The assay demonstrated simplicity, rapidity, and accuracy when tested against Staphylococcus aureus, Haemophilus influenzae, and Neisseria gonorrhoeae.

    Conclusions:

    • A practical and effective paper disc-based assay for penicillinase detection has been established.
    • This method offers a simple, rapid, and accurate approach for identifying penicillinase-producing bacteria in clinical and research settings.
    • The assay's ease of use and quick results make it a valuable tool for microbiology diagnostics.

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