Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative immunohistochemistry.

K Schauenstein, G Wick

    Acta Histochemica. Supplementband
    |January 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Quantitative immunohistochemistry relies on antibody titration or specific signal measurement. This study explores quantitative immunofluorescence (IF), detailing fluorochrome properties, embedding medium effects, and optimal illumination for accurate results.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Circulating microaggregates as biomarkers for the Post-COVID syndrome.

    IDCases·2024
    Same author

    Autoimmunity to HSP60 during diet induced obesity in mice.

    International journal of obesity (2005)·2016
    Same author

    Is autoimmunity a side-effect of interleukin 2 production?

    Immunology today·2014
    Same author

    Spontaneous autoimmune thyroiditis - a bird's eye view.

    Immunology today·2014
    Same author

    Lipids and lymphocyte function.

    Immunology today·2014
    Same author

    Pentraxin 3 (PTX3) plasma levels and carotid intima media thickness progression in the general population.

    Nutrition, metabolism, and cardiovascular diseases : NMCD·2014
    Same journal

    [The distribution of intermediate filaments in intracranial vessels in humans].

    Acta histochemica. Supplementband·1992
    Same journal

    [Malignant angioendotheliomatosis as a rare cause of cerebrovascular insufficiency].

    Acta histochemica. Supplementband·1992
    Same journal

    [The effect of arteriosclerosis on the wall elasticity of the human common carotid artery].

    Acta histochemica. Supplementband·1992
    Same journal

    [Vascular etiopathogenesis and degenerative dementias--current state of knowledge].

    Acta histochemica. Supplementband·1992
    Same journal

    Histochemical and morphometric investigation of the pathogenesis of acute brain infarction in primates.

    Acta histochemica. Supplementband·1992
    Same journal

    [Current aspects in diagnosis of Parkinson disease].

    Acta histochemica. Supplementband·1992
    See all related articles

    Area of Science:

    • Immunohistochemistry
    • Fluorescence Microscopy
    • Biomedical Imaging

    Background:

    • Quantitative data in immunohistochemistry can be obtained through antibody titration or by measuring the specific signal of marker molecules.
    • Quantitative immunofluorescence (IF) studies offer valuable insights into antigen-antibody interactions in histological preparations.

    Purpose of the Study:

    • To discuss theoretical aspects of quantitative immunofluorescence.
    • To analyze the impact of fluorochrome properties, embedding media, and illumination sources on fluorescence intensity.
    • To highlight practical considerations for accurate quantitative IF measurements.

    Main Methods:

    • Macrospectrofluorometric measurements were used to assess fluorescence properties.
    • Investigated the effects of embedding medium composition and dye concentration on emission intensity.

    Related Experiment Videos

  • Compared laser light excitation with filtered mercury arc lamps for illumination.

    • Main Results:

    • Embedding medium composition and dye concentration significantly affect fluorescence emission intensity.
    • Protein conjugation quenches UV-excited fluorescence but not fluorescence excited by visible blue light (496 nm).
    • Laser light excitation offers advantages over traditional mercury arc lamps for quantitative IF.
    • Demonstrated recovery of faded fluorescence with short, repeated laser light pulses.

    Conclusions:

    • Understanding fluorochrome properties and optimizing experimental conditions are crucial for accurate quantitative immunofluorescence.
    • Laser excitation and specific protocols can enhance the reliability and reproducibility of quantitative IF.
    • The findings have practical significance for improving quantitative immunohistochemical analyses.