Double labeling study of anionic sites and concanavalin A binding sites in monkey macrophages

Cationized ferritin (CF) binding, and its effect on the concanavalin A (Con A) binding pattern were studied by the double technique in monkey peritoneal macrophages. CF particles formed clumps and were internalized when cells were incubated at 37 degrees C. Such cells were fixed, and the Con A binding sites were visualized by the Con A-horseradish peroxidase (HRP) method. Using the same specimen, the distribution of CF particles and the Con A-HRP product was observed under an electron microscope. The redistribution and internalization of CF particles did not affect the continuous label of the cell surface Con A binding sites. These observations suggest the independent mobility of cell surface anionic sites and Con A binding sites.