Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mutation modifying the serine pathway in methylotrophic bacteria.

R Ratomahenina, P Galzy

    Folia Microbiologica
    |January 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Purification and Characterization of an Adipamidase from a Mutant Strain of Brevibacterium sp. Involved in Dinitrile Degradation.

    Bioscience, biotechnology, and biochemistry·2016
    Same author

    Natural nitriles and their metabolism.

    World journal of microbiology & biotechnology·2014
    Same author

    Study of the phenotypic and genotypic biodiversity of potentially ochratoxigenic black aspergilli isolated from grapes.

    International journal of food microbiology·2009
    Same author

    Glucose metabolism in the yeast Schwanniomyces castellii: role of phosphorylation site I and an alternative respiratory pathway.

    Applied and environmental microbiology·1997
    Same author

    Fatty hydroxamic acid biosynthesis in aqueous medium in the presence of the lipase-acyltransferase from Candida parapsilosis.

    Journal of biotechnology·1997
    Same author

    Contribution to the study of surface microflora on Cantal cheese.

    Acta microbiologica et immunologica Hungarica·1997

    Glycine inhibits the growth of methylotrophic bacteria. This study investigated its effects on key enzymes in Micrococcus varians and Bacillus licheniformis, finding specific inhibition of glycine-oxaloacetate aminotransferase.

    Area of Science:

    • Microbiology
    • Enzymology
    • Biochemistry

    Background:

    • Methylotrophic bacteria utilize the serine pathway for carbon assimilation.
    • Glycine, an amino acid, can impact bacterial metabolic processes.

    Purpose of the Study:

    • To investigate the inhibitory effects of glycine on methylotrophic bacteria growth.
    • To determine the impact of glycine on specific enzyme activities in Gram-positive bacteria.

    Main Methods:

    • Studying wild and mutant strains of Micrococcus varians and Bacillus licheniformis.
    • Assaying the activities of glycerate dehydrogenase, isocitrate lyase, serine hydroxymethyltransferase, and glycine-oxaloacetate aminotransferase.
    • Determining kinetic constants for inhibited enzymes.

    Related Experiment Videos

    Main Results:

    • Glycine at 0.5% inhibited the growth of methylotrophic bacteria.
    • Glycine-oxaloacetate aminotransferase (EC 2.6.1.35) was identified as an inhibited enzyme.
    • Kinetic constants were determined for glycine-oxaloacetate aminotransferase in two strain types.

    Conclusions:

    • Glycine exerts inhibitory effects on methylotrophic bacteria, specifically targeting glycine-oxaloacetate aminotransferase.
    • Understanding these enzymatic interactions is crucial for methylotrophic bacteria metabolism research.