Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multilayer film elements for clinical analysis: general concepts.

H G Curme, R L Columbus, G M Dappen

    Clinical Chemistry
    |August 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Examining the effects of prenatal alcohol exposure on performance of the sustained attention to response task in children with an FASD.

    Human brain mapping·2023
    Same author

    Syngas biomethanation: Study of process performances at high syngas flow rate in pressurized stirred column.

    Bioresource technology·2023
    Same author

    Syngas biomethanation: In a transfer limited process, is CO inhibition an issue?

    Waste management (New York, N.Y.)·2023
    Same author

    Examining the effects of prenatal alcohol exposure on corticothalamic connectivity: A multimodal neuroimaging study in children.

    Developmental cognitive neuroscience·2021
    Same author

    "Biomethanation of syngas by enriched mixed anaerobic consortium in pressurized agitated column".

    Bioresource technology·2021
    Same author

    Rapid inactivation of <i>Toxoplasma gondii</i> bradyzoites during formulation of dry cured ready-to-eat pork sausage.

    Food and waterborne parasitology·2020
    Same journal

    From Pooled Cohorts to PREVENT: A Perspective for Clinical Laboratorians.

    Clinical chemistry·2026
    Same journal

    Interlaboratory Comparison of a Glucagon and Oxyntomodulin Immuno-LC-MS/MS Assay: Implications for Diabetes Research.

    Clinical chemistry·2026
    Same journal

    Comparison of Information-Dependent Acquisition and Sequential Window Acquisition of All Theoretical Mass Spectra for Untargeted Drug Testing on a Linear Ion Trap-Pulsing Quadrupole-Time of Flight Mass Spectrometer.

    Clinical chemistry·2026
    Same journal

    Patterns of One-Year Change in HbA1c and Continuous Glucose Monitoring (CGM) Metrics in Older Adults with Type 2 Diabetes.

    Clinical chemistry·2026
    Same journal

    TSH Pediatric Reference Intervals: Lack of CALIPER Applicability to US-Based Populations.

    Clinical chemistry·2026
    Same journal

    Rapid Detection of Hemoglobinopathy Variants Using One-Step Library Preparation and Nanopore Sequencing.

    Clinical chemistry·2026
    See all related articles

    New dry film technology enables simple, accurate clinical analysis using colorimetry. This method precisely measures glucose in patient serum, offering a reliable alternative to existing reference methods.

    Area of Science:

    • Clinical chemistry
    • Analytical chemistry
    • Materials science

    Background:

    • Traditional clinical analysis often requires complex laboratory setups and multiple reagent steps.
    • There is a need for simplified, user-friendly diagnostic tools for point-of-care or decentralized testing.

    Purpose of the Study:

    • To develop and validate a novel dry film format for colorimetric clinical analysis.
    • To assess the performance of these dry films for quantifying specific analytes, such as glucose.

    Main Methods:

    • Designed dry films with reagents immobilized in a hydrophilic polymer matrix on a plastic base.
    • Incorporated a porous polymer spreading layer for uniform sample distribution and diffusion.
    • Utilized reflection densitometry with a linearized optical density function for quantification.

    Related Experiment Videos

  • Validated the method against a standard hexokinase reference method for glucose measurement.

    • Main Results:

    • The dry film format successfully initiated colorimetric reactions upon serum application.
    • The spreading layer ensured rapid, uniform fluid distribution and efficient analyte diffusion.
    • Demonstrated high precision for glucose measurement, with coefficients of variation as low as 1.5%.
    • Results for patient serum glucose concentrations closely correlated with the hexokinase reference method.

    Conclusions:

    • The developed dry film technology provides a robust and accurate platform for colorimetric clinical assays.
    • This format simplifies sample handling and analysis, making it suitable for various diagnostic applications.
    • The method shows significant promise for reliable and accessible glucose monitoring.