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Related Experiment Videos

Antigen B-specific helper factor from timothy grass pollen: isolation and partial characterization.

C J Brandt, L B Deppe, A Malley

    Immunology
    |October 1, 1981
    PubMed
    Summary

    Researchers identified a soluble antigen B-specific helper factor (THF) from mouse spleen cells. This factor aids T-cell activity and requires normal spleen cells to function, offering insights into immune responses.

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    Area of Science:

    • Immunology
    • Molecular Biology

    Background:

    • T-cell activity is crucial for adaptive immunity.
    • Understanding helper T-cell factors is key to modulating immune responses.

    Purpose of the Study:

    • To characterize a soluble helper factor (THF) secreted by antigen-primed T-cells.
    • To investigate the functional requirements and properties of THF.

    Main Methods:

    • Adoptive transfer of primed mouse spleen cells.
    • In vitro culture with antigen B and normal spleen cells.
    • Partial purification of THF using immobilized antigen adsorbent.
    • Molecular weight determination via SDS-PAGE.

    Main Results:

    • THF secretion was observed from T-cells stimulated with antigen B.

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  • THF demonstrated T cell-replacing activity, contingent on normal spleen cells.
  • Partially purified THF has a molecular weight of 65,000 Da and contains 5% carbohydrate.
  • THF is I-Ak-positive and Ig-negative.

    • Conclusions:

    • A soluble, antigen-specific helper factor (THF) with T cell-replacing activity has been identified.
    • THF's activity is dependent on accessory cells, suggesting complex immune interactions.
    • The characterized properties of THF provide a basis for further investigation into its role in T-cell help.