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Citrus artifact interference in aflatoxin M1 determination in milk.
Journal - Association of Official Analytical Chemists
|November 1, 1981
Summary
Citrus waste in dairy cow feed can cause false positives for aflatoxin M1 in milk using HPLC. Thin layer chromatography (TLC) offers a reliable alternative for accurate aflatoxin M1 detection.
Area of Science:
- Food Science
- Analytical Chemistry
- Dairy Science
Background:
- Dairy cows consuming feed contaminated with mycotoxins can excrete these toxins or their metabolites into milk.
- Accurate detection of mycotoxins like aflatoxin M1 in milk is crucial for food safety and public health.
Purpose of the Study:
- To investigate the effect of dried citrus waste, when fed to dairy cows, on the quantification of aflatoxin M1 in milk.
- To identify potential interferences in common analytical methods for aflatoxin M1 detection.
Main Methods:
- Dairy cows were fed dried citrus waste.
- Milk samples were analyzed for aflatoxin M1 using high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC).
Main Results:
- A compound present in dried citrus waste was found to be excreted into milk.
- This compound interfered with the HPLC analysis, leading to false positive results for aflatoxin M1.
- Thin-layer chromatography (TLC), with appropriate solvent selection, successfully overcame the interference and provided accurate quantification.
Conclusions:
- Dried citrus waste can cause analytical interference in aflatoxin M1 testing of milk via HPLC.
- TLC is a more reliable method for detecting aflatoxin M1 in milk from cows fed citrus waste, provided suitable developing solvents are used.