[Routine determination of Neisseria gonorrhoeae susceptibility to antibiotics by a disk diffusion method (author's...

The susceptibility of 137 isolates of Neisseria gonorrhoeae to eleven antibiotics has been determined by a disk diffusion method. For nine antibiotics (penicillin G, ampicillin, amoxicillin, cefazolin, cefoxitin, tetracycline, minocycline, erythromycin and chloramphenicol) the results were found to be similar to those of fast growing aerobic bacteria antibiogram procedures. The correlation between diameter of inhibition measured with chocolate agar 1 p. cent polyvitex BioMérieux and minimum inhibitory concentrations determined by an agar dilution procedure with GC medium 1 p. cent haemoglobin, 1 p. cent supplement B Difco was good : coefficients ranging from r = 0,78 for penicillin G to r = 0,60 for minocycline. Less satisfactory results were found if chocolate agar 1 p. cent polyvitex was substituted by Gono-Meningo medium IPP. The technique described could be used for clinical purposes and epidemiologic survey.