Effect of insulin on acetate metabolism in chicken adipocytes

The effects of insulin on the metabolism of acetate-U-14C in isolated chicken adipocytes have been investigated. Insulin added in vitro at physiological concentrations did not stimulate the incorporation of acetate-U-14C into triglycerides or CO2 when the acetate was present alone. In the presence of glucose 11m M, however, insulin stimulated significantly lipogenesis from acetate. This is an indirect action mediated by glucose.