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[Changes in phosphorylase activity during muscle alteration].

V I Stabrovskaia, A D Braun

    Tsitologiia
    |November 1, 1981
    PubMed
    Summary
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    Heating and urea decrease phosphorylase (PhL) activity in frog muscles. Electric stimulation, however, significantly increases active PhL (PhL a) levels during tetanic contractions.

    Area of Science:

    • Biochemistry
    • Muscle Physiology
    • Enzymology

    Context:

    • Skeletal muscle function is regulated by enzyme activity, particularly phosphorylase (PhL).
    • Understanding enzyme stability and activity under various stressors is crucial for muscle physiology research.
    • Investigating the impact of physical and chemical agents on enzyme kinetics provides insights into cellular mechanisms.

    Purpose:

    • To investigate the effects of heating, urea, and electric stimulation on phosphorylase (PhL) extractability, activity, and activation state in Rana temporaria skeletal muscle.
    • To compare the stability of PhL in isolated form versus within the intact muscle under different conditions.
    • To determine if PhL interconversion between inactive (PhL b) and active (PhL a) forms occurs during muscle contractures and stimulation.

    Summary:

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    • Heating and urea treatments reduced PhL extractability and overall activity in skeletal muscle, with isolated PhL showing greater thermostability than in situ. Urea only affected PhL at concentrations causing irreversible muscle damage, and isolated PhL was found to be highly labile to urea.
    • No conversion of inactive PhL b to active PhL a was observed during contractures induced by heating or urea.
    • Tetanic muscle contractions induced by electric stimulation resulted in a 2.5–3.0-fold increase in active PhL a activity, while total PhL activity remained constant.

    Impact:

    • This study reveals differential responses of phosphorylase to various stressors, highlighting the importance of the muscle's structural integrity for enzyme regulation.
    • Findings suggest that electrical stimulation is a potent physiological activator of PhL a, distinct from the effects of heat or urea.
    • The research contributes to understanding the complex mechanisms governing muscle energy metabolism and enzyme modulation during physiological and pathological conditions.