Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A rapid and sensitive solid-phase enzyme immunoassay for C-reactive protein.

E M Salonen

    Journal of Immunological Methods
    |January 1, 1982
    PubMed
    Summary

    A new enzyme immunoassay (EIA) allows rapid and sensitive measurement of C-reactive protein (CRP) in serum. This method accurately quantifies CRP levels within 4 hours, aiding in the diagnosis of inflammation and tissue injury.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Violence-related facial trauma: analysis of multidetector computed tomography findings of 727 patients.

    Dento maxillo facial radiology·2010
    Same author

    Multidetector computed tomography imaging of facial trauma in accidental falls from heights.

    Acta radiologica (Stockholm, Sweden : 1987)·2007
    Same author

    Anti-nucleosome antibodies in the diagnosis of systemic lupus erythematosus.

    Scandinavian journal of rheumatology·2005
    Same author

    Anti-telomere antibodies in systemic lupus erythematosus (SLE): a comparison with five antinuclear antibody assays in 430 patients with SLE and other rheumatic diseases.

    Annals of the rheumatic diseases·2004
    Same author

    Infections of the central nervous system of suspected viral origin: a collaborative study from Finland.

    Journal of neurovirology·2001
    Same author

    New method for coating tympanostomy tubes to prevent tube occlusions.

    International journal of pediatric otorhinolaryngology·2001

    Area of Science:

    • Biochemistry
    • Immunology
    • Clinical Chemistry

    Background:

    • C-reactive protein (CRP) is a key acute-phase reactant indicating inflammation or tissue injury.
    • Accurate and rapid quantification of CRP is crucial for clinical diagnostics.

    Purpose of the Study:

    • To develop a rapid and sensitive solid-phase enzyme immunoassay (EIA) for serum CRP determination.
    • To establish the performance characteristics of the developed EIA method.

    Main Methods:

    • Development of a solid-phase enzyme immunoassay (EIA) using polyethylene glycol (PEG).
    • Quantification of serum CRP within 4 hours at room temperature.
    • Determination of the minimum detectable CRP amount.

    Main Results:

    • The developed EIA method quantifies CRP within 4 hours at room temperature.
    • The minimum detectable CRP concentration was approximately 500 pg/ml.
    • Excellent correlation was observed between the solid-phase EIA and nephelometric assay, radial immunodiffusion, and latex agglutination tests.

    Conclusions:

    • The developed solid-phase EIA is a rapid, sensitive, and accurate method for serum CRP determination.
    • This EIA method shows excellent correlation with established CRP assays.
    • The assay is suitable for clinical settings requiring timely inflammation marker assessment.

    Related Experiment Videos