Collagen-bound glycoprotein of Guerin epithelioma

The polymeric collagen of Guerin epithelioma is strongly bound to a large amount of noncollagenous substance. Almost full dissociation of this complex was achieved by heating in 7 Murea, at 100 degrees C for 4 hours. The collagen bound substance was identified as an acidic glycoprotein containing glucose, galactose, glucosamine, galactosamine and M-acetylneuraminic acid. Heterogeneity of this substance in regard to molecular weight was found.