Perturbed angular correlation studies of 111In-labelled platelets

Indium-111, as the 8-hydroxyquinoline and acetylacetone complexes, has been used to label cellular blood components. Because of the gamma-ray cascade emitted following the decay of indium-111, perturbed angular correlation studies can be used to investigate the environment of the indium nucleus. Perturbed angular correlation studies have been carried out on the indium at various steps in the procedure used to label platelets with indium using both 8-hydroxyquinoline and acetylacetone as the ligand. Similar values of the integral correlation coefficient were obtained in both cases, suggesting identical labeling mechanisms. Following lysis of the platelets the values of (G22(infinity)) changed, suggesting that the intracellular complex is relatively weak.