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Updated: Aug 9, 2026

High-Throughput Measurement and Classification of Organic P in Environmental Samples
Published on: June 8, 2011
Interferences of mannitol with phosphate determination
Abstract:
Methods commonly used for serum phosphate analysis depend upon the combination of inorganic phosphorus with molybdate and subsequent quantitation of the resultant phosphomolybdate by spectrophotometry. This is a case report of mannitol interference with phosphate quantitation by a method not employing dialysis (DuPont ACA) but not with another method which includes a dialysis step (Technicon SMAC). This interference, occurring at an extremely high level of serum mannitol (3.5 g/dl) in a patient with acute renal failure, appears to be due to direct inhibition of the formation of the phosphomolybdate complex by mannitol.
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