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Interferences of mannitol with phosphate determination.

M T McCoy, J J Aguanno, S E Ritzmann

    American Journal of Clinical Pathology
    |April 1, 1982
    PubMed
    Summary
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    High serum mannitol levels can interfere with phosphate testing methods that do not use dialysis. This interference was observed in a patient with acute renal failure, impacting spectrophotometric analysis.

    Area of Science:

    • Clinical Chemistry
    • Analytical Chemistry
    • Biochemistry

    Background:

    • Serum phosphate analysis is crucial for diagnosing and monitoring various medical conditions.
    • Common methods involve reacting inorganic phosphorus with molybdate, followed by spectrophotometric quantitation.
    • Interference from other substances can affect the accuracy of these analyses.

    Observation:

    • A case report details a patient with acute renal failure and extremely high serum mannitol levels (3.5 g/dl).
    • Phosphate quantitation was affected by mannitol using the DuPont ACA method, which lacks a dialysis step.
    • The Technicon SMAC method, which includes a dialysis step, was not affected by the high mannitol levels.

    Findings:

    • Mannitol directly interferes with the formation of the phosphomolybdate complex.

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  • This interference specifically impacts phosphate analysis methods that do not incorporate a dialysis step.
  • The presence of high serum mannitol levels can lead to inaccurate phosphate measurements.

    • Implications:

    • Clinical laboratories should be aware of potential mannitol interference in phosphate assays.
    • Method selection is critical, favoring techniques with dialysis steps when high mannitol levels are suspected.
    • Accurate phosphate measurement is vital for managing patients, especially those with renal failure.