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Related Experiment Videos

Allotypes in Basilea rabbits.

S Weiss, I Garcia, J C Jaton

    European Journal of Immunology
    |February 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Basilea rabbits predominantly produce immunoglobulin with lambda light chains. Surprisingly, homozygous bas/bas rabbits can develop autoantibodies against b9 molecules, despite their presence in pre-immune samples.

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    Area of Science:

    • Immunology
    • Genetics
    • Animal Models

    Background:

    • Basilea rabbits are a unique strain characterized by a high prevalence of lambda light chains in their immunoglobulin molecules.
    • This variant strain originated from a homozygous (b9/b9) male, suggesting a genetic basis for the observed immunoglobulin characteristics.

    Purpose of the Study:

    • To investigate the presence and levels of b9 molecules in homozygous bas/bas rabbits.
    • To determine if these rabbits can produce autoantibodies against their own b9 molecules.

    Main Methods:

    • Utilized sensitive serological methods to detect and quantify b9 molecules in rabbit serum.
    • Administered pneumococcal and streptococcal vaccines to assess changes in b9 molecule levels upon hyperimmunization.
    • Generated and characterized anti-b9 allotypic antisera.

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    Main Results:

    • Homozygous bas/bas rabbits possess trace amounts of b9 molecules (approx. 100 ng/ml) in pre-immune bleeds.
    • Hyperimmunization led to an increase in b9 molecule levels, reaching up to 1 microgram/ml in most rabbits, with one exceptional case at 50 micrograms/ml.
    • Despite the presence of b9 molecules, homozygous bas/bas rabbits produced strong anti-b9 antibodies, indicating autoantibody production.

    Conclusions:

    • Homozygous bas/bas rabbits exhibit a unique immunoglobulin profile with a propensity for lambda light chains.
    • These rabbits are capable of producing autoantibodies against their own b9 allotypic molecules, even in the presence of these molecules prior to immunization.
    • The generated anti-b9 allotypic antisera were comparable to routinely produced ones, validating the findings.