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Spontaneous sweat secretion in calcium-free strontium medium
The American Journal of Physiology
|May 1, 1982
Summary
Strontium (Sr2+) can trigger sweat secretion in simian eccrine sweat glands, but requires higher concentrations than calcium (Ca2+). This Sr2+-induced secretion occurs spontaneously and is inhibited by various factors, suggesting specific cellular mechanisms.
Area of Science:
- Physiology
- Ion Channel Function
- Cellular Secretion
Background:
- Calcium (Ca2+) ions are crucial for regulating sweat secretion in eccrine glands.
- The role of strontium (Sr2+) as a Ca2+ substitute in sweat secretion is not fully understood.
Purpose of the Study:
- To investigate the effects of strontium (Sr2+) on sweat secretion in isolated simian eccrine sweat glands.
- To characterize the mechanisms underlying Sr2+-induced spontaneous sweat secretion (SSS).
Main Methods:
- Utilized isolated simian eccrine sweat glands.
- Administered methacholine (MCH) to evoke secretion.
- Investigated the effects of varying Sr2+ and Ca2+ concentrations.
- Assessed inhibition of SSS using atropine, ouabain, and D600, and by altering temperature.
Main Results:
- Sr2+ substitutes for Ca2+ in MCH-evoked sweat secretion, but requires 4-8 times higher concentrations.
- Ca2+-free Sr2+ medium significantly reduced the anticholinergic effect of atropine.
- Spontaneous sweat secretion (SSS) was observed in Sr2+ medium, saturating at 8 mM Sr2+.
- Sr2+-induced SSS was inhibited by Sr2+ removal, low temperature, ouabain, and D600.
- Ca2+ inhibited Sr2+-induced SSS, but with a non-unity slope on Schild plot.
Conclusions:
- Sr2+ can induce sweat gland secretion, mimicking some Ca2+ functions but with different potency.
- Sr2+-induced SSS involves active secretory processes.
- The data suggest Sr2+ enters cells via undefined membrane channels, triggering secretion.