Related Experiment Video

Updated: Jun 24, 2026

Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli
12:19

Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli

Published on: June 27, 2015

Bicarbonate reabsorption and potassium handling in isolated perfused kidneys of Bufo marinus

S Long

    The American Journal of Physiology
    |July 1, 1982
    PubMed

    More Related Videos

    Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium08:46

    Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

    Published on: September 1, 2015

    The Mouse Isolated Perfused Kidney Technique08:19

    The Mouse Isolated Perfused Kidney Technique

    Published on: November 17, 2016

    Related Concept Videos

    Drug Elimination by Renal Route: Tubular Reabsorption01:22

    Drug Elimination by Renal Route: Tubular Reabsorption

    During the process of renal excretion, as the glomerular filtrate progresses to the distal convoluted tubule (DCT), drugs that are highly permeable, lipophilic, and nonionized undergo passive reabsorption from the tubular fluid into the surrounding peritubular capillaries. This reabsorption process restricts their elimination through the kidneys. However, the majority of drugs are either weak acids or weak bases, and their ionization level is dependent on pH. By altering the pH of urine, the...
    Antihypertensive Drugs: Potassium-Sparing Diuretics01:28

    Antihypertensive Drugs: Potassium-Sparing Diuretics

    Liddle syndrome is a genetically inherited form of hypertension characterized by the overactivity of epithelial sodium channels in the nephron, the functional unit of the kidney. This heightened activity leads to increased sodium reabsorption and excessive excretion of potassium. To counteract this, potassium-sparing diuretics such as amiloride are used. They function by blocking these sodium channels, thereby reducing the influx of sodium into the epithelial cells and minimizing the loss of...
    Reabsorption and Secretion in the Loop of Henle01:17

    Reabsorption and Secretion in the Loop of Henle

    The thick ascending limb of the nephron loop has Na+–K+–2Cl− symporters in the apical membranes of its cells. These symporters simultaneously reclaim one sodium ion, one potassium ion, and two chloride ions from the tubular fluid. Sodium ions are actively transported into the interstitial fluid at the base and sides of the cell, diffusing into the vasa recta. Chloride ions move through leakage channels in the basolateral membrane into the interstitial fluid and then into the vasa recta.
    Renal Regulation of Acid-Base Balance01:29

    Renal Regulation of Acid-Base Balance

    Metabolic reactions in the body produce nonvolatile acids, such as sulfuric acid, which generate an acid load of approximately 1 mEq of H+ per kilogram of body weight daily. Excreting H+ in the urine is essential to balance this acid load.
    In the kidneys, cells within the proximal convoluted tubules (PCT) and the collecting ducts secrete hydrogen ions (H+) into the tubular fluid. Specifically, in the PCT, Na+/H+ antiporters secrete H+ while reabsorbing Na+.
    However, the intercalated cells in...
    Renal Drug Excretion: Effect of Urine pH, Flow Rate, and Drug pKa01:22

    Renal Drug Excretion: Effect of Urine pH, Flow Rate, and Drug pKa

    The pH of urine, the drug's pKa, and the urine flow rate are vital parameters for drug reabsorption and excretion. Urinary pH varies between 4.6 and 8.0 and is influenced by diet, drug intake, and the patient's pathophysiology. It affects a drug's ionization state and reabsorption. For instance, carbohydrate-rich food produces alkaline urine promoting drug excretion, while proteins and certain medications like ascorbic acid lead to acidic urine enhancing reabsorption.
    The pKa of a drug,...
    Physiology of the Genitourinary System II: Tubular Reabsorption and Secretion01:22

    Physiology of the Genitourinary System II: Tubular Reabsorption and Secretion

    The kidneys maintain homeostasis through filtration, reabsorption, and secretion. Tubular reabsorption and secretion are crucial in forming urine and regulating electrolytes, water balance, and waste elimination.Tubular Reabsorption and Secretion ProcessesTubular reabsorption is the process that reclaims essential substances such as electrolytes, glucose, amino acids, and water from the glomerular filtrate back into the bloodstream. This is achieved through passive and active transport...