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Updated: Aug 6, 2026

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
Published on: June 16, 2011
Abstract:
The use of in vitro transcription of polynucleotides by prokaryotic and eukaryotic DNA-dependent RNA polymerases has proven of great usefulness in detecting mutagenic base modifications. Quite similar results are obtained with ribo- and deoxyribopolymers as templates, and with Mg2+ and Mn2+ at optimal concentrations. Recent studies have centered on polymers containing etheno C, as well as its hydrated form. These modifications are of particular interest, since they are the consequence of metabolised vinyl chloride, a proven human carcinogen.
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