The distribution of sodium and potassium in toad (Bufo bufo) oocytes estimated by a stereological method

As the toad oocyte grows, the intracellular Na concentration rises and the K concentration falls. By stereological analysis using a point counting method in a projection apparatus, it is shown that as the oocyte grows the volume density of the nucleus, endoplasmic reticulum and mitochondria falls, while that of the yolk platelets and cytoplasmic vesicles rises. A multiple regression analysis applied to the sodium and potassium concentrations and to the volume densities of the subcellular components indicates that sodium is most concentrated in cytoplasmic vesicles while potassium is most concentrated in a component composed of the cell cytoplasmic ground substance, the mitochondria and the cell nucleus.