Immunochemical demonstration of different antigens in single cells in paraffin-embedded histological sections

A new modification of the double staining immunochemical technique is described, which is capable of demonstrating the presence of several antigens in single cells at the light microscopical level. This method was employed by the authors in order to detect the simultaneous occurrence of different types of cytoplasmic immunoglobulin chains in neoplastic plasma cell precursors in paraffin-embedded lymph-node specimens from patients with B-cell malignancies.