[Method of controlling live vaccines against listeriosis]

Used was Anton's conjunctival test with guinea pigs to control the actual immunogenicity and innocuity of a live vaccine against listeriosis. It was found that local immunity could be produced following a successive conjunctival infection only, initially with the use of a listeriosis vaccine and in not less than ten days later with pathogenic strains of the respective serotype. The method makes it possible to carry out both titration and control on the equal volume ratio of the vaccinal strains of the two serotypes in the vaccine.