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Updated: Aug 26, 2026

Functional Complementation Analysis (FCA): A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways
Published on: June 24, 2016
Cell genetic studies on propionyl-CoA carboxylase deficient cell lines
Abstract:
Thirteen propionyl-CoA carboxylase (PCC)-deficient fibroblast lines were analysed for complementation by examining the incorporation of [14C]propionate into trichloroacetic-acid insoluble cellular macromolecules of polyethylene-glycol-dimethyl-sulphoxide induced heterokaryons formed by fusion of mutant cell lines. Corrections for variations in the rate of protein synthesis of the heterokaryons and individual cell lines was made by measuring the incorporation of [3H]lysine, added simultaneously with the labelled propionate. Two distinct complementation groups were found in this sample of PCC-deficient cell lines. Evidence for intragenic complementation was not obtained.
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