Related Experiment Videos
A poly(U)-binding factor stimulating EF-1 activity in the wheat-germ soluble fraction
European Journal of Biochemistry
|March 15, 1983
Summary
A novel poly(U)-binding factor in wheat embryos stimulates elongation factor 1 (EF-1). This factor copurifies with EF-1L but can be separated by heat, which inactivates EF-1 while preserving poly(U)-binding activity.
Area of Science:
- Plant molecular biology
- Protein biochemistry
Background:
- Poly(U)-binding proteins play roles in RNA regulation.
- Elongation factor 1 (EF-1) is crucial for protein synthesis.
Purpose of the Study:
- To identify and characterize a poly(U)-binding factor in wheat embryos.
- To investigate the relationship between this factor and EF-1.
Main Methods:
- Wheat embryo homogenization and fractionation.
- Protein purification using Sephadex G-200, DEAE-cellulose, hydroxyapatite, and poly(U)-Sepharose chromatography.
- Heat treatment to separate factors.
Main Results:
- A poly(U)-binding activity was detected in wheat embryo extracts.
- This activity copurified with EF-1L, the lighter form of EF-1.
- Heat treatment separated the poly(U)-binding activity from EF-1, inactivating EF-1 but not the binding activity.
Conclusions:
- Wheat embryos contain a distinct poly(U)-binding factor that stimulates EF-1.
- This factor is separable from EF-1 and may have independent regulatory functions in translation.