Effect of the substratum on the growth of CFU-c in continuous marrow culture

The effect of the substratum on the maintenance of granulocyte-macrophage progenitors (CFU-c) was studied in continuous mouse marrow culture. Glass and decalcified eggshell membrane were found preferable to plastic, providing better adherence of stromal cells. Eggshell membrane consists of a meshwork of branching fibers suitable for the adherence of stromal cells. The glass surface with its high surface pH may provide an electrostatic attraction for the negatively charged surface of stromal cells.