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Neural crest ablation and limb morphogenesis.

R Shoobridge, D Velkou, J McCredie

    The Journal of Experimental Zoology
    |January 1, 1983
    PubMed
    Summary
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    Ablating chick embryo neural crest cells impacted leg development, with deformities linked to dorsal root ganglia size, not spinal cord area. This suggests neural crest influences limb morphogenesis.

    Area of Science:

    • Developmental biology
    • Neuroscience
    • Embryology

    Background:

    • The neural crest is a transient embryonic structure crucial for development.
    • Understanding the neural crest's role in limb development is essential for regenerative medicine.

    Purpose of the Study:

    • To investigate the impact of early caudal neural crest ablation on chick embryo limb development.
    • To determine the relationship between neural crest, neural tube, and limb morphogenesis.

    Main Methods:

    • Diathermy ablation of left caudal neural crest in 2-day-old chick embryos.
    • Examination of neural crest, neural tube, somitic mesoderm, and limb development at different time points.
    • Analysis of dorsal root ganglia and spinal cord morphology in surviving chicks.

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    Main Results:

    • Ablation caused damage extending into the neural tube and somitic mesoderm.
    • Chicks with limb deformities showed reduced dorsal root ganglia mass but normal spinal cord area.
    • Chicks without limb deformities had reduced spinal cord but not dorsal root ganglia.

    Conclusions:

    • Limb morphogenesis appears quantitatively related to neural crest presence, but not the neural tube.
    • Experimental limitations necessitate further investigation with improved methods.