Variants of type-C retroviruses from DBA/2 mice: protein-structural and biological properties

Ecotropic murine leukemia viruses isolated from normal and carcinogen-treated DBA/2 mice can be classified into three main groups that differ in structure and biology. Two groups, called Ea and Eb, consist of N-tropic viruses related to the standard endogenous ecotropic virus of AKR mice. Ea viruses replicate with reduced efficiency in cell lines derived from C3H mice, while Eb viruses essentially replicate normally in these cells. As elsewhere reported, Ea viruses appear apathogenic in C3H mice, while Eb viruses cause a moderate incidence of late leukemias. The biological differences are associated with modulations of the fine structure of the gag gene-encoded proteins. A third group of viruses, called Ec, is clearly more diverged. They differ extensively from Ea and Eb viruses in the products of the gag and env gene, and are related to Rauscher leukemia virus. Ec viruses are NB-ecotropic; they replicate efficiently in all mouse cells tested, and induce leukemias in C3H mice with shorter latency periods than Eb viruses. Since published nucleic acid hybridization data indicate that DBA/2 mice only carry one ecotropic provirus, we assume that the DBA/2 viruses represent a developmental series of variants evolving during the life of the animals.