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Related Experiment Videos

A new fluid switching flow sorter.

J Dühnen, J Stegemann, C Wiezorek

    Histochemistry
    |January 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a novel capsular flow cytometer sorter to mitigate risks associated with conventional cell sorting. The new design enhances safety by enclosing the sorting mechanism, preventing hazardous microdroplet and microbial contamination.

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    Area of Science:

    • Biotechnology
    • Cell Biology
    • Analytical Chemistry

    Background:

    • Conventional cell sorters generate hazardous microdroplets containing toxic dyes and radiolabeled compounds.
    • These substances pose risks, including potential mutagenicity and carcinogenicity, to personnel operating the equipment.
    • Separating living cells in an open stream risks microbial contamination, affecting subsequent cell cultures.

    Purpose of the Study:

    • To develop a safer cell sorting method.
    • To eliminate the risks of hazardous microdroplet expulsion and microbial contamination.
    • To create a contained cell sorting system for improved laboratory safety.

    Main Methods:

    • Construction of a novel capsular flow cytometer sorter.
    • The new design features a small chamber that fully encases the sorting mechanism.

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  • Utilized a microcomputer-based pulse height analyzer for data acquisition, analysis, and processing.

    • Main Results:

    • The capsular flow cytometer sorter effectively contains microdroplets and prevents aerosol contamination.
    • The enclosed system significantly reduces personnel exposure to hazardous substances.
    • The microcomputer-based system ensures accurate data handling for cell sorting.

    Conclusions:

    • The developed capsular flow cytometer sorter provides a safer alternative to conventional methods.
    • This innovation minimizes health risks and contamination concerns in cell sorting applications.
    • The system is suitable for sorting living cells for further study without compromising sample integrity.