Measurement of the translational diffusion constant of G-actin by photon correlation spectroscopy

Photon correlation spectroscopic measurements on monomeric actin have yielded a translational diffusion constant of 8.13 X 10(-7) cm2 s-1 after correction for the contribution of the back-reflections of the main beam. This value corresponds to a sphere with a radius of 2.6 nm and 50% hydration, or to a prolate ellipsoid with axes 2.0 and 4.0 nm and 30% hydration.