Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phase separation and lens cell age.

J I Clark, J R Neuringer, G B Benedek

    Journal of Gerontology
    |May 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Age-related changes in lens cytoplasm cause phase transitions, leading to opacity. X-irradiation alters this process, potentially causing cataracts by shifting the characteristic temperature (Tcat) of phase separation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Overall survival by clinical risk category for high dose interleukin-2 (HD IL-2) treated patients with metastatic renal cell cancer (mRCC): data from the PROCLAIM<sup>SM</sup> registry.

    Journal for immunotherapy of cancer·2019
    Same author

    Immune checkpoint inhibitors in advanced renal cell carcinoma: experience to date and future directions.

    Annals of oncology : official journal of the European Society for Medical Oncology·2017
    Same author

    Multidisciplinary treatment of olfactory neuroblastoma: Patterns of failure and management of recurrence.

    American journal of otolaryngology·2015
    Same author

    Axitinib versus sorafenib in advanced renal cell carcinoma: subanalyses by prior therapy from a randomised phase III trial.

    British journal of cancer·2014
    Same author

    Role of cytokines in pathology of melanoma and use of biologic response modifiers in melanoma.

    Methods in molecular medicine·2012
    Same author

    Measurement of the Velocity of Blood Flow (in vivo) Using a Fiber Optic Catheter and Optical Mixing Spectroscopy.

    Applied optics·2010
    Same journal

    Shakespeare's attitude towards old age.

    Journal of gerontology·2010
    Same journal

    Involution of tissues in fetal life; a review.

    Journal of gerontology·2010
    Same journal

    Attitudes toward aging and the aged; primitive societies.

    Journal of gerontology·2010
    Same journal

    Budgeting for social security.

    Journal of gerontology·2010
    Same journal

    The Hodson Community Center; an experiment in preservation of personality.

    Journal of gerontology·2010
    Same journal

    Prolongation of life with prevention of leukemia by thymectomy in mice.

    Journal of gerontology·2010
    See all related articles

    Area of Science:

    • Ophthalmology
    • Biophysics
    • Cell Biology

    Background:

    • Age-related changes in lens cytoplasm are studied using laser light scattering spectroscopy.
    • Cataract development is linked to phase transitions in lens cytoplasm, causing protein concentration differences and light scattering.

    Purpose of the Study:

    • To investigate age-related microstructural changes in lens cytoplasm.
    • To understand the biochemical basis for altered phase transition temperatures (Tcat) in X-irradiated lens cells during aging.

    Main Methods:

    • Laser light scattering spectroscopy was used to study lens cytoplasm microstructure.
    • Animal models were employed to observe age-related changes and the effects of X-irradiation.

    Main Results:

    Related Experiment Videos

    • A phase transition in lens cytoplasm is an early indicator of cataract development.
    • In normal aging, Tcat decreases in nuclear cells. X-irradiation increases Tcat with age, leading to cataracts near body temperature.

    Conclusions:

    • Lens cytoplasm phase separation is a key mechanism in age-related cataract formation.
    • X-irradiation significantly alters the aging process of lens cells, influencing Tcat and cataract development.