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Corticosterone binding sites along the rat nephron.

S M Lee, M A Chekal, A I Katz

The American Journal of Physiology

|May 1, 1983

Summary

Glucocorticoid receptors are concentrated in the rat kidney's cortical collecting tubule. This finding identifies key nephron segments targeted by corticosterone, influencing kidney function.

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Area of Science:

Nephrology
Endocrinology
Molecular Biology

Background:

Glucocorticoids are vital for kidney function.
The specific locations of glucocorticoid receptors within the nephron remain largely unknown.
Understanding receptor distribution is crucial for elucidating glucocorticoid action in the kidney.

Purpose of the Study:

To precisely map the distribution of glucocorticoid receptors in the rat nephron.
To identify the specific nephron segments that bind corticosterone, the primary endogenous glucocorticoid in rats.

Main Methods:

Microdissection of discrete rat nephron segments from adrenalectomized rats.
Measurement of [3H]corticosterone binding to these isolated segments.
Quantification of specific binding capacity at 25 degrees C.

Main Results:

Highest corticosterone binding was observed in the cortical collecting tubule.
Significant binding was also found in the distal convoluted tubule and medullary collecting tubule.
Binding was lowest in the thick ascending limb of Henle's loop, with minimal levels in the proximal tubule and pars recta.
Cortical collecting tubule binding capacity for corticosterone was significantly higher than for aldosterone.

Conclusions:

Specific binding sites for corticosterone are present throughout the rat nephron.
The cortical collecting tubule is the primary target site for corticosterone in the kidney.
These findings highlight the importance of the cortical collecting tubule in mediating glucocorticoid effects on kidney function.

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