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[Effect of macrophage and tumor cell fixation on their immunogenicity and antigenicity in an H-2 system]
A short-term fixation of macrophage monolayers by 0.002-0.25% glutaraldehyde solutions gives rise to inhibition of the specific adherence of allogeneic immune cytotoxic lymphocytes to treated monolayers, but does not prevent the absorption of anti-H-2 antibodies. Sarcoma cells fixed by a 0.25% glutaraldehyde or a 3% formaldehyde lost their capacity to induce cytotoxic lymphocytes, rosette forming cells or antibodies in allogeneic mice. The induction of the secondary response by fixed tumor cells was significantly reduced. The results fit well a conception on non-identity of determinants recognized by T cell receptors and serologically defined H-2 specificities.
A short-term fixation of macrophage monolayers by 0.002-0.25% glutaraldehyde solutions gives rise to inhibition of the specific adherence of allogeneic immune cytotoxic lymphocytes to treated monolayers, but does not prevent the absorption of anti-H-2 antibodies. Sarcoma cells fixed by a 0.25% glutaraldehyde or a 3% formaldehyde lost their capacity to induce cytotoxic lymphocytes, rosette forming cells or antibodies in allogeneic mice. The induction of the secondary response by fixed tumor cells was significantly reduced. The results fit well a conception on non-identity of determinants recognized by T cell receptors and serologically defined H-2 specificities.