Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural defects in DNA from primary hepatocyte cultures.

R D Moir, G J Smith, B W Stewart

    Cell Biology International Reports
    |March 1, 1983
    PubMed
    Summary

    Primary hepatocytes show significant DNA structural changes compared to intact liver cells. This DNA damage appears inherent to the primary cell state, not an artifact of isolation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Multi-decadal geochemical evolution of drainage from underground coal mines in the Appalachian basin, USA.

    The Science of the total environment·2024
    Same author

    Phosphorus and iron-oxide transport from a hydrologically isolated grassland hillslope.

    Journal of environmental management·2022
    Same author

    A single column separation method for barium isotope analysis of geologic and hydrologic materials with complex matrices.

    Geochemical transactions·2021
    Same author

    Effect of combining pre-exercise carbohydrate intake and repeated short sprints on the blood glucose response to moderate-intensity exercise in young individuals with Type 1 diabetes.

    Diabetic medicine : a journal of the British Diabetic Association·2019
    Same author

    Dietary protein affects both the dose and pattern of insulin delivery required to achieve postprandial euglycaemia in Type 1 diabetes: a randomized trial.

    Diabetic medicine : a journal of the British Diabetic Association·2018
    Same author

    A 10-second sprint does not blunt hormonal counter-regulation to subsequent hypoglycaemia.

    Diabetic medicine : a journal of the British Diabetic Association·2017

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Hepatology

    Background:

    • Primary hepatocytes are crucial for studying liver function and disease.
    • Understanding DNA integrity in primary cells is vital for reliable experimental outcomes.
    • Previous studies have not fully characterized DNA structural integrity during the transition to primary culture.

    Purpose of the Study:

    • To investigate structural alterations in DNA isolated from primary hepatocytes.
    • To compare DNA structure in primary hepatocytes with that of DNA from intact liver.
    • To determine if observed DNA structural changes are artifacts of the isolation procedure.

    Main Methods:

    • DNA isolation from primary hepatocytes and intact liver.
    • Chromatography using benzoylated-DEAE-cellulose.
    • In vivo labeling of DNA with 3H-thymidine (3H-TdR).

    Main Results:

    • DNA from primary hepatocytes exhibited increased binding to benzoylated-DEAE-cellulose in 1.0 M NaCl.
    • 32% of primary hepatocyte DNA was recovered in the formamide-eluted fraction.
    • Extensive shearing or procedural modifications did not alter this proportion.
    • DNA from hepatocyte cell lines showed structural similarity to intact liver DNA.

    Conclusions:

    • Primary hepatocytes display significant DNA structural alterations compared to DNA from intact liver.
    • These structural changes are likely a consequence of the primary cell status.
    • The observed DNA structural damage is not an artifact of the isolation method.

    Related Experiment Videos