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Evaluation of a chromatographic method for globin chain biosynthesis in thalassemia
Clinical Biochemistry
|June 1, 1983
Abstract:
We report our experience with a column chromatographic procedure for separating 3H-leucine-labelled alpha and beta globin. In non-thalassemic subjects the alpha: beta biosynthesis ratio was 1.04 +/- 0.10 S.D. An abnormal ratio was useful in defining thalassemia variants. The technique, although labour intensive, is not difficult and is recommended for any hospital laboratory acting as a reference centre for the diagnosis of hemoglobinopathies.