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Related Experiment Videos

Reflectance digital matrix photometry.

W E Neeley, A Zettner

    Clinical Chemistry
    |June 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    A new digital matrix photometer accurately quantifies light-absorbing substances in small colored spots. This innovative device uses stepwise scanning for precise measurements of analytes in areas as small as 1 mm.

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    Area of Science:

    • Analytical Chemistry
    • Instrumentation Science

    Background:

    • Quantitative measurement of small chromophoric areas is challenging.
    • Existing methods may lack precision for irregular or small samples.

    Purpose of the Study:

    • To design and construct a digital matrix photometer.
    • To enable accurate and precise quantitative measurement of reflected light from small colored spots.

    Main Methods:

    • Developed a digital matrix photometer utilizing a photodiode array.
    • Employed stepwise scanning to measure reflected light from small subunits of the target area.
    • Summated individual reflectance measurements for total analyte quantification.

    Main Results:

    • The digital matrix photometer allows for quantitative measurement of reflected light.

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  • Accurate and precise quantitation of light-absorbing substances was achieved.
  • The technique is effective for small areas (≥1 mm) with varying sizes, shapes, and internal irregularity.

    • Conclusions:

    • The developed digital matrix photometer is a viable tool for precise analyte quantification in small samples.
    • This method overcomes limitations in measuring irregular or small chromophoric areas.
    • The technology offers advancements in analytical chemistry for small-scale sample analysis.