Comparison of adhesion to glass and aggregation of human leucocytes

C5adesArg-induced leucocyte aggregation and neutrophil adhesion to glass were compared using the same cell separation technique and identical chemicals for preparing the media. Human serum albumin decreased adhesion to glass but had no effect on leucocyte aggregation induced by C5adesArg. In contrast, C5adesArg in Gey's solution with or without HSA, in serum or plasma stimulated leucocyte aggregation in a dose-dependent fashion. However, it did not alter neutrophil adhesion to glass. This indicates that leucocyte aggregation and neutrophil adherence to glass do not necessarily represent equivalent responses taking place on different substrata. Thus they may be mechanistically distinct.