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Functional prothrombinase complex assembly on isolated monocytes and lymphocytes.

P B Tracy, M S Rohrbach, K G Mann

    The Journal of Biological Chemistry
    |June 25, 1983
    PubMed
    Summary
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    Mononuclear cells, including monocytes and lymphocytes, generate thrombin, a key clotting protein, by interacting with coagulation Factors Va and Xa. Monocytes exhibit higher thrombin generation rates than lymphocytes, suggesting a role in inflammatory processes and fibrin deposition.

    Area of Science:

    • Coagulation cascade
    • Cellular immunology
    • Hemostasis

    Background:

    • Peripheral blood monocytes and lymphocytes interact with coagulation factors.
    • These cells form a catalytic complex to activate prothrombin to thrombin.

    Purpose of the Study:

    • To investigate the kinetics of prothrombin activation by monocytes and lymphocytes.
    • To determine the role of these cells in thrombin generation and potential implications in inflammation.

    Main Methods:

    • Continuous monitoring of prothrombin activation using a fluorescent thrombin inhibitor.
    • Incubation of cells with prothrombin, Factor Va, and Factor Xa in the presence of Ca2+.
    • Quantification of Factor V using radioimmunoassay and bioassay.

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    Main Results:

    • Monocytes and lymphocytes generate thrombin at rates exceeding those of platelets.
    • Monocyte thrombin generation is higher than lymphocytes and increases with monocyte adhesion.
    • Monocytes have approximately 16,100 Factor Va binding sites and intracellular Factor V.
    • Lymphocytes contain approximately 24,500 molecules of Factor V per cell.

    Conclusions:

    • Mononuclear cells, particularly monocytes, possess significant prothrombinase activity.
    • Factor Va acts as a receptor for Factor Xa on the monocyte surface.
    • Intracellular Factor V in mononuclear cells may be released at inflammatory sites, contributing to extravascular fibrin deposition and inflammation.